The third order rate constant for the nitrosation of the thiols by isopropyl nitrite and the equilibrium constant for the formation of isopropyl nitrite were found to be in good agreement with the literature values obtained by direct measurement. A similar kinetic study of the nitrosation of L cysteine steroids steroids, L cysteine methyl and ethyl esters, N acetyl L cysteine, thioglycolic acid and glutathione by various alkyl nitrites, in water at 25 C, in the pH range 6 13 was undertaken. The pH dependence of the rate constant is consistent with a mechanism involving a direct nitrosation by alkyl nitrites with the thiolate anion (RS(^ )) of the thiol.
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